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Professor David Greening

BSc(Hons) | PhD, University of Melbourne

Professor David Greening

Baker Institute Fellow

+61 3 8532 1111

Professor Greening is Head of Molecular Proteomics at Baker Heart and Diabetes Institute. He is the Helen Amelia Hains Fellow and Baker Institute Fellow, in addition to Head of the Baker Institute's Proteomics Research Platform. Prof Greening is Director of the Proteomics and Metabolomics Platform at La Trobe University, and group leader at Baker Department of Cardiovascular Research, Translation and Implementation, La Trobe University. He holds affiliate positions at the School of Translational Medicine, Monash University and Baker Department of Cardiometabolic Health, University of Melbourne.

The laboratory has recognised expertise in extracellular vesicle (EV) biology and acellular therapeutics, multi-omics, cell/mouse models of cardiac disease, aptamer biology applied to EVs, systems biology strategies to understand heart signalling, and comprehensive mass spectrometry analysis of EVs (including newly defined EVs and their functions). Prof Greening’s team is recognised for their expertise in cellular remodelling and cardiovascular disease, proteomic technologies, spatial development of proteomics applied to the heart, and extracellular biology and therapeutics. His international recognition in EVs — in strategies to decipher their form and function/bioactivity — and innovative proteomic technologies to understand mechanistic underpinnings of complex biology (regulation of complex cell-specific processes) and develop therapies that impact human disease.

He has established ties with national and international industry partners Tithon Biotech, Takeda Pharma, and Thermo Fisher Scientific, including significant engagement as international key opinion leader with various industry networks in biotechnology/proteomics and regenerative medicine fields. Prof. Greening has authored 148 papers with >24,500 career cites (>19,800 in the past 5 years) and a H-index of 52, including articles in top journals: Nature Methods, Nature Rev. Clinical Oncology, Circulation Research, Circulation, Nature Cardiovascular Research, Journal of Extracellular Vesicles, Blood, Nature Communications, Journal of Clinical Investigation, and Molecular Cellular Proteomics. He is co-founder/past-President of the Australian & New Zealand Society for Extracellular Vesicles (ANZSEV), and key advocate for the development and integration of EV science and knowledge in the region. He has led international efforts in conference organisation and management, including International Society of Extracellular Vesicles (Chair, ISEV2024 (Aus)), international organising committees for ISEV2022, ISEV2023, and organising committee of Biomolecular Horizons 2024 Congress (Aus). Prof Greening has been awarded Australian Field Leader (top rank) in proteomics since 2020, and recipient of several prestigious awards, including ROYAN International Research Award on Stem Cell Biomedicine, International Protein Society Hans Neurath Outstanding Promise Award, and Ludwig Institute for Cancer Research Excellence Medal.

Research overview

The Molecular Proteomics laboratory is focused on understanding the molecular function of nano-sized extracellular vesicles and how their intercellular signalling is important in heart physiology and cardiac pathologies, with the goal of identifying new deliverable therapeutic targets.

We use a multi-disciplinary approach to understand the molecular function of extracellular vesicles incorporating proteomics, cell biology, molecular biology, nanobiotechnology, functional assays, cell and animal models, with the goal of understanding mechanisms of cell signalling and function, identifying deliverable therapeutic targets and engineering nanoparticles for next generation cell-free therapies.

  • Bioengineered extracellular vesicles for cardiovascular therapeutics.
  • Engineering and targeted delivery of biological extracellular vesicles.
  • Extracellular vesicles: diagnostic platform for cardiovascular disease.
  • Barcoding circulating extracellular vesicles — understanding organ-specific intercellular signalling.
  • Repairing a broken heart: exosomes in cardiac regeneration.
  • Demystifying what makes up a heart using subcellular and spatial proteomics.

Achievements

  • Helen Amelia Hains Fellow and Baker Fellow
  • President, Australia and New Zealand Society of Extracellular Vesicles
  • Australian Field Leader (outright) – Proteins, Proteomics (Australian Research Magazine)
  • Chair, International Society of Extracellular Vesicles Congress (2024)
  • Highly Cited Researchers 2020 (top 1%) by Stanford University citation impact (2021)
  • Research Excellence Scholarship, American Journal of Physiology
  • International Protein Society Hans Neurath Outstanding Promise Award
  • ROYAN International Research Award on Reproductive Biomedicine

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With the rising number of Australians affected by diabetes, heart disease and stroke, the need for research is more critical than ever.

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